In vitro propagation In vitro propagation of Dahlia sp. using different types of explants and plant growth regulators (PGR)

In vitro propagation of Dahlia sp.

Abstract

Dahlia sp., a family of Compositeae has potential value as an alternative food and medicinal plant. Its tubers contain carbs, fiber, and many other beneficial substances. Dahlia sp. can be propagated via in vitro culture to create uniform seeds, free from pests and pathogens, more stable, and can be scale up in a short time. The addition of cytokinin and auxins in the media can stimulate the shoot formation of Dahlia sp planlets. This research aimed to determine the growth response of Dahlia sp. cultures on DKW (Driver and Kuniyuki Walnut) medium supplemented with BAP, kinetin, 2-iP, and NAA and to identify the best type of explant used for the micropropagation of Dahlia sp. The experimental design was a completely randomized factorial design, with factors tested: type of explant (apical shoots, middle and basal nodes), and plant growth regulator combination between cytokinins (BAP, Kinetin, and 2-iP ) and auxin (NAA) at 0 and 1 mg/L. The result demonstrated that  the DKW medium supplemented with 1 mg/L 2-iP  and 1 mg/L NAA using middle nodes as explants produced the highest values for shoot height, number of shoots, number of roots, fresh weight, and dry weight variables. Apical shoots explant cultured on DKW medium supplemented with 1 mg/L Kinetin and 0 mg/L NAA produced the highest number of leaves and were significantly different from the others.